antiprmt5  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: PRMT5 as an Epigenetic Target for Group 3 (MYC-driven) Medulloblastoma

doi: 10.64898/2026.04.09.717536

Figure Lengend Snippet: (A) qPCR analysis for the expression of PRMT5/MYC mRNA in two MYC-amplified cell lines with transiently knocked-down of PRMT5 (using siRNAs) at 72 h. ****, p<0.0001 (student t test, SCR vs siPRMT5). (B) Effect of PRMT5 knockdown (siRNAs) on MYC-luciferase reporter gene (MYC-Luc) activity in HD-MB03 cells. *, p<0.05 (student t test) (C) ChIP analyses for the enrichment of PRMT5 and H4R3me2s on the proximal promoter region of the MYC gene in HD-MB03 cells. ****, p<0.0001 (student t test). ChIP analyses for the enrichment/binding of PRMT5 (D) and H4R3me2s (E) to the proximal promoter region of MYC gene, in PRMT5 knockdown (siRNAs) HD-MB03 cells. ( F ) Co-immunoprecipitation of BRD4 with PRMT5. ( G ) ChIP analyses for the enrichment/binding of PRMT5 and BRD4 to the proximal promoter region of MYC gene, in BRD4 knockdown (siRNAs) HD-MB03 cells. *, p<0.05; **, p<0.05 (student t test).

Article Snippet: Four PRMT5 inhibitors (EPZ015666, GSK332595, LLY-283, JNJ64619178) were purchased from MedChemExpress LLC or Selleckchem Company.

Techniques: Expressing, Amplification, Knockdown, Luciferase, Activity Assay, Binding Assay, Immunoprecipitation

Journal: bioRxiv

Article Title: PRMT5 as an Epigenetic Target for Group 3 (MYC-driven) Medulloblastoma

doi: 10.64898/2026.04.09.717536

Figure Lengend Snippet: RNA-sequencing was used to assess global gene expression changes in HD-MB03 cells 24 h after treatment with DMSO (vehicle) or JNJ64619178 (1 µM). (A) Volcano plot displaying genes significantly upregulated or downregulated in response to PRMT5 inhibition. (B) Gene Ontology Biological Process (GO-BP) functional analysis for top 10 pathways altered by JNJ. (C) Gene sets enrichment analysis (GSEA) (with p<0.01 and FDR<0.2) for top 10 pathways/gene sets (including MYC-associated target gene sets, RNA splicing, metabolism) altered by PRMT5 inhibition. (D) Number of the aberrant splicing events in protein coding genes disrupted by PRMT5 inhibition. (E) GO-BP functional analysis for the aberrant splicing events altered by PRMT5 inhibition. (F) Volcano plot represents all the significant (p<0.05) splicing events. Selected genes from the indicated GO-BP functional classification are highlighted. Metabolism associated genes GGT6, GPT2 and C1QBP1 are identified.

Article Snippet: Four PRMT5 inhibitors (EPZ015666, GSK332595, LLY-283, JNJ64619178) were purchased from MedChemExpress LLC or Selleckchem Company.

Techniques: RNA Sequencing, Gene Expression, Inhibition, Functional Assay

Journal: bioRxiv

Article Title: PRMT5 as an Epigenetic Target for Group 3 (MYC-driven) Medulloblastoma

doi: 10.64898/2026.04.09.717536

Figure Lengend Snippet: (A) MTT assay showing the effects of PRMT5 inhibitors (1-50 µM) on cell growth of MYC-amplified MB (HD-MB03, D341), non-MYC MB (ONS-76) and normal human astrocyte (NHA) cell lines. (B) IC 50 values of PRMT5 inhibitors in the indicated cell lines. (C) Annexin-V assay showing effects of PRMT5 inhibitors (JNJ, EPZ) on apoptosis in HD-MB03 cells. **, p<0.01, ***, p<0.001 (relative to ‘0’ (vehicle control)). (D) Cell cycle profile in HD-MB03 cells treated with PRMT5 inhibitors (EPZ, JNJ). (E) Western blot analysis for the expression of the indicated key proteins in JNJ-treated HD-MB03 cells. ( F) Quantification of spheres following treatment of JNJ and EPZ in two PDX-derived MB cell lines (MED-411FH, MED-114FH) at 72 h. Values, mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.005; *** p < 0.001 (Student- t -test). (G) Representative sphere (MED-411FH) images showing disruption of spheres in each treatment. (H) Western blot results showing the expression of indicated proteins in MED-411 spheres treated with JNJ and EPZ.

Article Snippet: Four PRMT5 inhibitors (EPZ015666, GSK332595, LLY-283, JNJ64619178) were purchased from MedChemExpress LLC or Selleckchem Company.

Techniques: MTT Assay, Amplification, Annexin V Assay, Control, Western Blot, Expressing, Derivative Assay, Disruption

Journal: bioRxiv

Article Title: PRMT5 as an Epigenetic Target for Group 3 (MYC-driven) Medulloblastoma

doi: 10.64898/2026.04.09.717536

Figure Lengend Snippet: (A ) JNJ brain concentration in BALB/c mice (N=3) following oral administration of 10 mg/kg JNJ at different timepoints. **p<0.01 (Student’s t-test). (B) NSG mice (N=5) with subcutaneously xenografted HD-MB03 cells were treated orally with vehicle or JNJ (10 mg/kg) five times a week for three weeks. Tumor volume measurement of xenografted mice following treatments. The differences noted between treatment groups show comparison by Student ‘s t-test of the tumor volumes on 21 days post treatment (p<0.005). (C) Representative IHC images (40 × magnification with 60 µm scale bar) of PRMT5, MYC, Ki-67, and CC3 expression in xenografts 21 days post-treatment, as indicated. Bar graphs below show the percentages of PRMT5, MYC, Ki-67 and CC3 positive cells derived from immunohistology scores, which were semi-quantitated in the tumors of three xenografted mice. **p<0.01; ***p<0.005 (Student’s t-test). (D) NSG mice (N=6) with orthotopically xenografted HD-MB03 cells were treated daily with vehicle or JNJ (10 mg/kg) or JNJ (F) (10 mg/kg) for two weeks. Survival analysis of xenografted mice using Kaplan-Meier (long-rank test). *p<0.05, **p<0.01, ***p<0.001. (E) Representative IHC images (4x magnification with 600 µm scale bar) and respective quantification showing MYC-positive tumors in the mouse cerebellum. The percentage of MYC, derived from immunohistology scores, was semi-quantitated in the tumors of three xenografted mice 21 days post-treatment. *p< **p<0.01 (Student’s t-test).

Article Snippet: Four PRMT5 inhibitors (EPZ015666, GSK332595, LLY-283, JNJ64619178) were purchased from MedChemExpress LLC or Selleckchem Company.

Techniques: Concentration Assay, Comparison, Expressing, Derivative Assay